Transgenic Core services offered to TAMUS Faculty
TIGM had completed its move from Houston to College Station in August 2010. We have the personnel, expertise, facilities and equipment to provide mouse transgenic core services for the Texas A&M System community. Those services are offered to our TAMUS colleagues at cost. For more information about the services TIGM can offer you please email Dr. Andrei Golovko at: agolovko@tigm.org.
Form to Transfer Animals Between TAMUS facilities
Animal Transfer Form (doc)
Prices effective November 1, 2023
Basic Pronuclear/Cytoplasmic Injection (including CRISPR/Cas9*)
$3,564
Investigator provides at least 30 ug of digested plasmid DNA, detailed map of the construct and a picture of the DNA with the desired band circled. In case of CRISPR, investigator provides uncut plasmid vector expressing sgRNA, sequences of the sgRNA and template DNA vector of oligo as needed. Includes injection of at least 125 FVB embryos. All resulting offspring will be transferred to the investigator at weaning. TIGM can also genotype the offspring for the transgene at an extra cost.
Pronuclear Injection, other strains (C57BL/6, etc.)
Basic Pronuclear Injection + Additional $835
Pronuclear Injection of BAC DNA
Basic Pronuclear Injection + Additional $835
Please contact the core to obtain protocols for preparing BAC DNA. BAC DNA needs to be submitted to the core as a pellet under 70% ethanol.
*Listed rates apply to indels and point mutations only. For more complex projects (conditional knockouts and knock-ins) please contact us at info@tigm.org to obtain a detailed quote.
[More details] [Download Form]
Blastocyst Injection
$2,599
Investigator provides two vials of the frozen clone. The service includes injection of at least 30 blastocysts/clone. All resulting offspring will be transferred to the investigator at weaning.
[More details] [Download Form]
Sperm Cryopreservation
$619
Sperm from two male mice will be cryopreserved using the protocol from the Jackson Laboratory. The cryopreservation fee includes sperm viability testing by in vitro fertilization. After the first year there is a biannual $110 liquid nitrogen storage fee.
[More details] [Download Form]
Embryo Cryopreservation
$1,733
Investigator transfers 6-8 practiced stud males, 3-6 months of age, to TIGM quarantine. The core will perform two sessions and will cryopreserve at least 200 embryos at the morula stage. Additional sessions can be performed at a cost of $880. The cryopreservation fee includes embryo viability testing. After the first year there is a biannual $110 liquid nitrogen storage fee.
[More details] [Download Form]
Rederivation via IVF (live mice)
$1,671
Investigator transfers 2-3 practiced stud males, 3-6 months of age, to TIGM quarantine for sperm collection. The core will isolate oocytes from at least 5 superovulated females (C57BL/6N, C57BL/6J or CRD1 ICR strains; other strains could be used too, but additional charges related to animal order may apply), perform IVF and transfer the resulting embryos to recipient females. All resulting offspring will be transferred to the investigator at weaning. The remaining sperm sample can be cryopreserved and stored at TIGM. After the first year there is a biannual $110 liquid nitrogen storage fee.
[More details] [Download Form]
Rederivation via IVF (sperm samples prepared using Jax/Nakagata protocol)
$1,361
Investigator provides at least 2 straws each containing at least 40-50 ul of frozen sperm. The core will isolate oocytes from at least 5 superovulated females(C57BL/6N, C57BL/6J or CRD1 ICR strains; other strains could be used too, but additional charges related to animal order may apply), perform IVF and transfer the resulting embryos to recipient females. All resulting offspring will be transferred to the investigator at weaning.
[More details] [Download Form]
Rederivation via IVF (sperm samples prepared using other protocols)
$3,960
Investigator provides at least 4 straws/vials each containing approximately 200 ul of frozen sperm. The core will perform two sessions and will use oocytes from 20 donors per vial of sperm. C57BL/6N, C57BL/6J or CRD1 ICR strains could be used as donors; other strains could be used too, but additional charges related to animal order may apply.The remaining straws will be kept in reserve for another transfer attempt if necessary. All resulting offspring will be transferred to the investigator at weaning.
[More details] [Download Form]
Rederivation via Embryo Transfer
$1,733
Investigator transfers 3-6 practiced stud males, 3-6 months of age, to TIGM quarantine for mating with superovulated females. The core will perform 1-2 sessions and will transfer at least 100 embryos at the morula stage.
[More details] [Download Form]
Frozen Embryo Transfer
$507
Investigator provides at least 4 straws each containing approximately 20 embryos. 2 straws will be used for transfer. The remaining straws will be kept in reserve for another transfer attempt if necessary. All resulting offspring will be transferred to the investigator at weaning.
[More details] [Download Form]
Live Embryo Transfer
$507
Investigator provides early stage embryos in M2 medium or alternatively freshly isolated uterine or oviduct tissues from timed pregnancies for the core to flush. The investigator must coordinate with the core at least a week in advance so that the core can set up recipient females at the appropriate stage. All resulting offspring will be transferred to the investigator at weaning.
[More details] [Download Form]
Colony maintenance
$1.24 per cage/day
Includes general husbandry, health monitoring, breeding, weaning, ear tagging, tail clipping.
[More details] [Download Form]
Genotyping (per sample)
1 PCR reaction $6
2 and more PCR reactions $12
Colony maintenance
$1.69 per cage/day
Includes general husbandry, health monitoring, breeding, weaning, ear tagging, tail clipping and genotyping as needed.
[More details] [Download Form]
Live mice from stock colony
C57BL/6N, C57BL/6J, FVB, Balb-c male or female: $19 per mouse
CD1 ICR male or female: $12 per mouse
Pregnant female, timed pregnancy (C57BL/6N, C57BL/6J, FVB, Balb-c): $39 per mouse
Setting up for timed matings/checking plugs: no cost if the mice are provided and housed at TIGM
Gene Targeting via Homologous Recombination (constitutive, conditional or knock-in)
Services include:
- Targeting vector construction and designing of the screening strategy
- Transfection of ES cells and confirmation of targeted clones
- ES cell blastocyst injection
- Chimera Breeding
- Genotyping protocol design and confirmation
Services can be requested individually or as a package. Prices may vary depending on complexity of a project. Please contact TIGM for more information.
Electroporation service package
$4,208
The service includes linearization/purification/ electroporation of targeting construct; isolation/expansion of drug-resistant colonies in 96-well format (at least 2 plates); preparation of cell pellet plates in 96-well format and expansion of all targeted ES cell clones as specified by the customer.
Additional services
Identification of targeted ES cell clones by PCR $1,361 per plate
Expansion of targeted ES cell clones $294 per clone
[More details] [Download Form]
Prices reflect a 10% discount from regular academic rates for TAMUS colleagues.
